• Runx also controls the proliferation and neuronal differenti

  2018-11-08

  

  Runx1 also controls the proliferation and neuronal differentiation of certain neural progenitor cell populations. In embryonic olfactory bulb progenitor cells in vivo, as well as in cultures of either embryonic olfactory progenitor cells or embryonic cortical NSPCs, Runx1 increases cell proliferatio

• 69 8 Finally we cultured hiPSCs according to the present

  2018-11-08

  

  Finally, we cultured hiPSCs according to the present strategy with some modifications (Figure 4A; Supplemental Experimental Procedures). hiPSCs formed colonies on day 0. In the mesoderm induction phase, these colonies were disrupted, but the 69 8 generated some clusters upon CHIR and Cyc treatment.

• Using the combination of a mitogen activated protein

  2018-11-08

  

  Using the combination of a mitogen-activated protein kinase kinase (MEK) inhibitor, PD0325901 (PD03), and a glycogen synthase kinase 3 (GSK3) inhibitor, CHIR99021 (CHIR), which will hereafter be referred to as 2i, mouse ESCs (mESCs) are maintained in a ground state (Ying et al., 2008). CHIR activate

• br Results br Discussion Direct

  2018-11-08

  

  Results Discussion Direct reprogramming of human somatic RG-108 into functional neurons has been proposed as an alternative strategy to obtain patient-specific neurons for disease modeling, drug screening, and toxicity tests (Qiang et al., 2014). Indeed, this approach offers some advantages a

• Subsequent differentiation of stem cell derived MK

  2018-11-08

  

  Subsequent differentiation of stem cell-derived MK progenitors into mature MKs and ultimately platelets has been notoriously difficult. Coculture with OP9 or C3H10T1/2 stroma cystic fibrosis chloride channel has been found to greatly facilitate the process (Lu et al., 2011; Takayama et al., 2008),

• br Materials and methods br Acknowledgements

  2018-11-08

  

  Materials and methods Acknowledgements This work was supported by grants from Instituto de Salud Carlos III (PI 15/000227 to MC, Plataforma en Red de Recursos Biomoleculares y BioinformaticosPRB2 PT13/0001/0042) cofunded by FEDER. Resource table. Resource details Loss-of-function mutation

• br Materials and methods br Results br Discussion Several in

  2018-11-08

  

  Materials and methods Results Discussion Several in vivo and in vitro studies indicate that OPCs have substantially different properties according to their chronological age. For example, human fetal OPCs from different gestational stages exhibit differences in the myelination profile (Cui

• In proof of principle experiments

  2018-11-08

  

  In proof-of-principle experiments, we examined the response of hESC-derived CMs to verapamil and isoproterenol, two drugs commonly used in cardiovascular research. Single CMs exhibited a significantly reduced calcium transient amplitude associated with a negative inotropic response to treatment with

• It is interesting that OB neurons produced from the

  2018-11-08

  

  It is interesting that OB neurons produced from the transient Gli1-enriched subcallosal domain in juvenile mice mostly corresponded to sGCs, when they were derived from a region with active SHH signaling. It is possible that the relative levels of SHH signaling are adjusted in different domains. The

• Next we attempted to correct the disease causing

  2018-11-08

  

  Next we attempted to correct the disease-causing mutation in a fibroblast line isolated from an infant with severe combined immunodeficiency (SCID), caused by mutations in the gene encoding adenosine deaminase (ADA). SCID patients could particularly benefit from a one-step protocol that facilitates

• We have explored the possibility of using the cardiac

  2018-11-08

  

  We have explored the possibility of using the cardiac MSCs of the human fetal heart for expansion of LDN193189 Hydrochloride with characteristics similar to those of mesenchymal progenitors that contribute to mammalian cardiogenesis. In this study cardiac MSCs were reproducibly recovered from the a

• To gain further insight into progenitor cells derived

  2018-11-08

  

  To gain further insight into progenitor xpo 1 derived from human skeletal muscle, we performed comprehensive cell-surface protein profiling of two progenitor populations, CD56+ myogenic progenitors and PDGFRα+ mesenchymal progenitors. This comprehensive analysis identified previously unrecognized m

• Flow cytometry analysis showed that independent

  2018-11-07

  

  Flow cytometry analysis showed that independent targeted clones expressed remarkably similar levels of the inserted reporter genes, highlighting the consistency of expression produced by this vector configuration (Figure 1C). This consistency also enabled us to conclude that all the selectable marke

• br Conclusions The results indicate that in general chances

  2018-11-07

  

  Conclusions The results indicate that, in general, chances in the Brazilian budgetary institutions have contributed to a better primary result of the federal government, particularly those changes captured by the indices that evaluate the budget categories and stages IC and IS and the sub-indices

• If cesarean delivery has a prolonged effect

  2018-11-07

  

  If cesarean delivery has a prolonged effect on the microbiota, this could contribute to the risk for metabolic diseases later in life. Herein, we explored whether the fecal microbiota differs between adults who reported that they were born by cesarean versus vaginal delivery. For comparison, in the

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